Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Coding

Part:BBa_K1218006

Designed by: Trevor Kalkus, Gordon Wade, Alissa Greenberg   Group: iGEM13_Stanford-Brown   (2013-08-28)

HisC E. coli (Modern)

HisC is a gene that makes a phosphate aminotransferase essential to the production of the amino acid histidine. Histidine does not grow abiotically, so this part was characterized in a pUC19 expression vector by rescuing HisC knockout strains grown on M9 minimal plates using IPTG as an inducer. For detailed pictures and growth curves, please go to the Stanford-Brown 2013 iGEM wiki page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None